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Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
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Bioengineering
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JoVE Journal Bioengineering
Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

DOI:
10.3791/60399-v

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10:27 min

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November 07, 2019

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, , , ,
1Department of Mechanical Engineering, Graduate School,Chung-Ang University, 2Department of Biomedical Engineering, College of Medicine,Seoul National University

Chapters

  • 00:05Title
  • 00:28Centrifugal Microfluidic-Based Spheroid (CMS) Culture Chip Fabrication
  • 02:06Cell Preparation
  • 03:10Cell Staining
  • 03:45Monoculture Spheroid Formation
  • 05:33Co-Culture Spheroid Formation: Concentric Spheroid Formation
  • 06:28Co-Culture Spheroid Formation: Janus Spheroid Formation
  • 07:33Co-Culture Spheroid Formation: Sandwich Spheroid Formation
  • 08:48Results: Representative CMS Culture Chip Spheroid Culture Analyses
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

We present a motor-powered centrifugal microfluidic device that can cultivate cell spheroids. Using this device, spheroids of single or multiple cell types could be easily cocultured under high gravity conditions.

Tags

Lab-on-a-CD PlatformMulticellular Three-dimensional SpheroidsCentrifugal ForceMicrowellsPDMSPlasma CleaningCell CultureCell DetachmentCell Labeling

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