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Journal / Biology / 超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法…
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超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法
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超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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该协议描述了CryoAPEX方法，其中APEX2标记膜蛋白可以通过传输电子显微镜在最佳保存的细胞超结构内进行局部化。

Tags

生物学， 问题 156， CryoAPEX， 膜蛋白， APEX2， 传输电子显微镜， 低温， 高压冷冻， 冷冻替代
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