Journal
/
/
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
DOI:

07:44 min

May 22, 2020

, , , , , , ,

Chapters

  • 00:04Introduction
  • 00:35Preparing Solutions and Plates (Day 1)
  • 01:50Blocking and Sample Addition (Day 2)
  • 03:42Antibody Addition
  • 05:17Reading the Plate
  • 06:10Results: Accuracy and Consistency of MeCP2-ECLIA
  • 07:20Conclusion

Summary

Automatic Translation

The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.

Related Videos

Read Article