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Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
DOI:

10:38 min

June 27, 2008

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Chapters

  • 00:00Title
  • 00:51Introduction
  • 02:04Dissection and Isolation of Early Hematopoietic Stem Cells
  • 07:19Dissociation and FACS Analysis
  • 09:57Conclusion

Summary

Automatic Translation

This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.

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