Obtaining Eggs from Xenopus laevis Females

Biology

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Summary

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.

Cite this Article

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Cross, M. K., Powers, M. Obtaining Eggs from Xenopus laevis Females. J. Vis. Exp. (18), e890, doi:10.3791/890 (2008).

Abstract

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.

Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

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Disclosures

The authors have nothing to disclose.

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