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Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
JoVE Journal
Biology
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JoVE Journal Biology
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
DOI:

13:48 min

September 07, 2008

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Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:21Preparing Transfections
  • 05:35Inducing Membrane Biosynthesis via Phagocytosis of Latex Beads
  • 08:07Performing the Dual-Glo Luciferase Assays
  • 11:28Data Analysis
  • 13:00Conclusion

Summary

Automatic Translation

Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-GloTM Luciferase Assay System from Promega.

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