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JoVE Journal Developmental Biology
Rettet differensiering av Primitive og Definitive blodkreft Progenitors fra menneskelige Pluripotent stamceller
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Rettet differensiering av Primitive og Definitive blodkreft Progenitors fra menneskelige Pluripotent stamceller

Article DOI: 10.3791/55196-v • 14:37 min • November 1st, 2017
November 1st, 2017 •
1, 1
1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

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Her presenterer vi menneskelige pluripotent stamceller (hPSC) kultur protokoller, som brukes til å skille hPSCs i CD34+ blodkreft progenitors. Denne metoden bruker scenen-spesifikke manipulering av kanoniske WNT signalisering til å angi celler eksklusivt til enten definitive eller primitive blodkreft programmet.

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Utviklingspsykologi biologi problemet 129 Pluripotent stamceller menneskelige embryonale stamceller cellekultur embryoid organer WNT signalnettverk definitiv hematopoiesis primitive hematopoiesis hemogenic endotelet
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