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JoVE Journal Neuroscience
Organotypiske Retinal Explant Kulturer fra Macaque Monkey
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Journal (Neuroscience)
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Collection of Cerebrospinal Fluid from Rat Cisterna Magna During EEG Recordings (Video) | JoVE
Journal (Neuroscience)
Collection of Cerebrospinal Fluid from Rat Cisterna Magna During EEG Recordings (Video) | JoVE
 
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Organotypiske Retinal Explant Kulturer fra Macaque Monkey

Article DOI: 10.3791/64178-v • 10:32 min • August 24th, 2022
August 24th, 2022 •
1, 1, 1, 1, 2, 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

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Retinal explants hentet fra villtype makaker ble dyrket in vitro. Netthinnegenerasjon og cGMP-PKG-signalveien ble indusert ved bruk av PDE6-hemmeren zaprinast. cGMP-akkumulering i eksplantene ved forskjellige zaprinastkonsentrasjoner ble verifisert ved bruk av immunfluorescens.

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