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A型流感病毒的核蛋白复合物的分离纯化及可视化
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Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Please note that all translations are automatically generated.
Click here for the English version.
A型流感病毒的核蛋白复合物的分离纯化及可视化
DOI:
10.3791/1105-v
•
09:35 min
•
February 09, 2009
•
Winco W.H. Wu
,
Lindsay L. Weaver
,
Nelly Panté
1
Department of Zoology
,
University of British Columbia – UBC
Chapters
00:00
Title
00:58
Introduction
01:27
Disruption of the Influenza A Virion
02:53
Glycerol Gradient Sediment Velocity Centrifugation
04:18
Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
05:40
Concentration of the vRNP Fractions
06:39
Negative Staining of vRNPs
08:25
Representative Results/ Outcome
09:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
A型流感病毒的基因组由8个独立的复合物,RNA和蛋白质,称为病毒核蛋白复合物(vRNPs)。本文介绍的甘油梯度纯化和透射电子显微镜A型流感vRNPs可视化。
Tags
Purification
Visualization
Influenza A
Viral Ribonucleoprotein Complexes
VRNPs
Viral Envelope
Nuclear Import
Replication
Isolated VRNPs
Procedure
Disruption
Detergents
Glycerol Gradient
Velocity Sedimentation
SDS-PAGE
Coomassie Blue Staining
Peak Fractions
Concentration
Centrifugation
Integrity Verification
Transmission Electron Microscopy
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