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A型流感病毒的核蛋白复合物的分离纯化及可视化
JoVE Journal
Biology
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JoVE Journal Biology
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
DOI:

09:35 min

February 09, 2009

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Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:27Disruption of the Influenza A Virion
  • 02:53Glycerol Gradient Sediment Velocity Centrifugation
  • 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
  • 05:40Concentration of the vRNP Fractions
  • 06:39Negative Staining of vRNPs
  • 08:25Representative Results/ Outcome
  • 09:05Conclusion

Summary

Automatic Translation

A型流感病毒的基因组由8个独立的复合物,RNA和蛋白质,称为病毒核蛋白复合物(vRNPs)。本文介绍的甘油梯度纯化和透射电子显微镜A型流感vRNPs可视化。

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