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量化老化细胞生长的酵母年谱跨度
JoVE Journal
Biology
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JoVE Journal
Biology
Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
Please note that all translations are automatically generated.
Click here for the English version.
量化老化细胞生长的酵母年谱跨度
DOI:
10.3791/1156-v
•
12:24 min
•
May 06, 2009
•
Christopher Murakami
,
Matt Kaeberlein
1
Department of Pathology
,
University of Washington
Chapters
00:05
Title
01:07
Introduction
01:33
Preparation of Aging Cultures
03:03
Taking a Viability Age-Point
04:43
Loading the Bioscreen C MBR Machine
06:26
Data Analysis
10:59
Representative Results
11:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在酵母老化的年代,是指与固定相相关的细胞活力的损失。在这里,我们描述了一个高通量的方法,定量确定酵母按时间顺序寿命。
Tags
Yeast
Chronological Lifespan
Aging Research
Replicative Aging
High-throughput Method
Quantitative Measurement
Defined Medium
Agitation
Constant Temperature
Aging Culture
Rich Growth Medium
Growth Curve
Bioscreen C MBR Machine
Algorithm
Viable Cells
Reproducibility
Precision
Genetic Screens
Chemical Screens
Longevity Modifiers
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