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Biology
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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
 

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Article DOI: 10.3791/1205
April 17th, 2009

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Summary April 17th, 2009

We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

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