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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Article DOI: 10.3791/1205 • 11:57 min • April 17th, 2009
April 17th, 2009 •
1, 2, 2, 1, 2
1Department of Evolutionary Functional Genomics, Evolutionary Biology Center, Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences

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Summary
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We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.

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Non-radioactive In Situ Hybridization Protocol Norway Spruce Plant Species Expression Analysis Technologies Expression Profiles Tissue Specific Expression In Situ Hybridization MRNA Expression Laborious Time-consuming Optimization Woody Species Conifer Picea Abies Modified DIG In Situ Hybridization Protocol RNase Treatment Proteinase K Concentration Homologous Genes Male Reproductive Organs Gymnosperm Angiosperm Species Arabidopsis Thaliana Brassica Napus Floral Organs Microsporophylls
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