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Generatie van de Mens CD40-geactiveerde B-cellen
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Human CD40-activated B cells
DOI:

13:27 min

October 16, 2009

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Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:46Preparation of Feeder Cells
  • 02:27Preparation of PBMC for CD40- Stimulation
  • 04:38Preparation of Feeder Cells
  • 08:33CD40- Stimulation
  • 09:50Subcultivation
  • 12:20Analysis of CD40- Activated B Cells
  • 12:55Conclusion

Summary

Automatic Translation

In deze video presenteren we de<em> Ex vivo</em> Generatie en de uitbreiding van de menselijke CD40-geactiveerde B-cellen (CD40-B) uit perifeer bloed mononucleaire cellen (PBMC) door stimulatie met CD40 ligand en interleukine-4.

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