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人CD40激活的B细胞的生成

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Biology
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JoVE Journal Biology

Generation of Human CD40-activated B cells

Please note that all translations are automatically generated. Click here for the English version.

人CD40激活的B细胞的生成

DOI:

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13:27 min

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October 16, 2009

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Tanja M. Liebig, Anne Fiedler, Shahram Zoghi, Alexander Shimabukuro-Vornhagen, Michael S. von Bergwelt-Baildon

¹Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program,University Hospital of Cologne, Department I of Internal Medicine

Chapters

00:00Title
00:14Introduction
01:46Preparation of Feeder Cells
02:27Preparation of PBMC for CD40- Stimulation
04:38Preparation of Feeder Cells
08:33CD40- Stimulation
09:50Subcultivation
12:20Analysis of CD40- Activated B Cells
12:55Conclusion

Summary

Automatic Translation

在这个视频中，我们目前的<em>体外</em>的产生和扩大人CD40激活的B细胞（CD40 - B）从外周血单个核细胞（PBMC）的刺激与CD40配体和IL - 4。

Tags

CD40-activated B Cells Cancer Immunotherapy Antigen-presenting Cells MHC Co-stimulatory Molecules Interleukin-4 CD40 Ligand Lymph Node Homing Triad Peripheral Blood In Vitro Expansion Cell Culture Cyclosporin A Recombinant Human CD40 Ligand

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