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Journal
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Biology
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人CD40激活的B细胞的生成
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Biology
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JoVE Journal
Biology
Generation of Human CD40-activated B cells
Please note that all translations are automatically generated.
Click here for the English version.
人CD40激活的B细胞的生成
DOI:
10.3791/1373-v
•
13:27 min
•
October 16, 2009
•
Tanja M. Liebig
,
Anne Fiedler
,
Shahram Zoghi
,
Alexander Shimabukuro-Vornhagen
,
Michael S. von Bergwelt-Baildon
1
Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program
,
University Hospital of Cologne, Department I of Internal Medicine
Chapters
00:00
Title
00:14
Introduction
01:46
Preparation of Feeder Cells
02:27
Preparation of PBMC for CD40- Stimulation
04:38
Preparation of Feeder Cells
08:33
CD40- Stimulation
09:50
Subcultivation
12:20
Analysis of CD40- Activated B Cells
12:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频中,我们目前的<em>体外</em>的产生和扩大人CD40激活的B细胞(CD40 - B)从外周血单个核细胞(PBMC)的刺激与CD40配体和IL - 4。
Tags
CD40-activated B Cells
Cancer Immunotherapy
Antigen-presenting Cells
MHC
Co-stimulatory Molecules
Interleukin-4
CD40 Ligand
Lymph Node Homing Triad
Peripheral Blood
In Vitro Expansion
Cell Culture
Cyclosporin A
Recombinant Human CD40 Ligand
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