JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
ヒトCD40活性化B細胞の生成
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Generation of Human CD40-activated B cells
Please note that all translations are automatically generated.
Click here for the English version.
ヒトCD40活性化B細胞の生成
DOI:
10.3791/1373-v
•
13:27 min
•
October 16, 2009
•
Tanja M. Liebig
,
Anne Fiedler
,
Shahram Zoghi
,
Alexander Shimabukuro-Vornhagen
,
Michael S. von Bergwelt-Baildon
1
Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program
,
University Hospital of Cologne, Department I of Internal Medicine
Chapters
00:00
Title
00:14
Introduction
01:46
Preparation of Feeder Cells
02:27
Preparation of PBMC for CD40- Stimulation
04:38
Preparation of Feeder Cells
08:33
CD40- Stimulation
09:50
Subcultivation
12:20
Analysis of CD40- Activated B Cells
12:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このビデオでは、提示<em>生体外で</emヒトCD40活性化B細胞(CD40 - B)CD40リガンドとインターロイキン-4で刺激による末梢血単核細胞(PBMC)からの>生成と展開。
Tags
CD40-activated B Cells
Cancer Immunotherapy
Antigen-presenting Cells
MHC
Co-stimulatory Molecules
Interleukin-4
CD40 Ligand
Lymph Node Homing Triad
Peripheral Blood
In Vitro Expansion
Cell Culture
Cyclosporin A
Recombinant Human CD40 Ligand
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
染色体分析のためのヒト胚性幹細胞の染色体スプレッドの準備
StemgentヒトTFのレンチセットでヒト線維芽細胞を再プログラムすることによって誘導多能性幹細胞の生成
ヒト胚性幹細胞集団から肝細胞様細胞の堅牢な生成
ヒト臍帯血由来血管内皮コロニー形成細胞の表現型および機能解析
Multititreプレートフォーマットでヒト多能性幹細胞から神経細胞の迅速かつ効率的な生成
シングル細胞におけるゼブラフィッシュセグメンテーション時計のイメージングのための分散前体節中胚葉細胞培養物の世代
ヒト心筋細胞の生成:フィーダーフリーヒト人工多能性幹細胞からの分化プロトコル
仙台ウイルスのヒト体細胞からの効率的な次世代ヒューマン誘導多能性幹細胞
ヒト末梢血からの血液伸長内皮細胞の生成と文化
トマト毛状根の発生によるDNAの修飾のハイスループットCRISPRベクター構築と特性
Read Article