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Journal
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Immunology and Infection
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取得高纯度
弓形虫
卵囊
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Obtaining Highly Purified
Toxoplasma gondii
Oocysts by a Discontinuous Cesium Chloride Gradient
Please note that all translations are automatically generated.
Click here for the English version.
取得高纯度
弓形虫
卵囊
DOI:
10.3791/1420-v
•
11:17 min
•
November 03, 2009
•
Sarah E. Staggs
,
Mary Jean See
,
J P. Dubey
,
Eric N. Villegas
4
1
Dynamac, Inc.
,
2
Department of Biological Sciences
,
University of Cincinnati, McMicken College of Arts and Science
,
3
Animal Parasitic Disease Laboratory
,
Agricultural Research Service, U.S. Department of Agriculture
,
4
National Exposure Research Laboratory
,
US Environmental Protection Agency
Chapters
00:00
Title
01:05
Introduction
01:35
General Safety When Working With T. gondii Oocysts
03:15
Protocol Preparation and the Sucrose Float
07:27
CsCl Gradient
10:03
Resulting Oocysts
10:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项研究描述了修改后的铯的方法，可以轻松地净化适用于分子生物学和组织培养操作感染的猫粪的弓形虫卵囊的发展
Tags
Toxoplasma Gondii
Oocysts
Cesium Chloride Gradient
Protozoan Pathogen
Food-borne Disease Outbreaks
Feline Species
Sexually Replication
Infectious Oocyst
Contaminated Meat
Contaminated Soil
Contaminated Water
Latent Infection
Toxoplasmic Encephalitis
Immunocompromised
Seroprevalence
Waterborne Outbreaks
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