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Chapters
- 00:00Title
- 00:09Introduction
- 00:30Viral Transduction of Mouse Embryonic Fibroblasts
- 01:25Doxycycline Induced Reprogramming
- 03:18Immunocytochemical Analysis of Transduction Efficiency
- 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
- 07:39Immunocytochemical Analysis for Pluripotency
- 09:14Pluripotency Marker Analysis
- 10:31Conclusion
Den Stemgent Dox inducerbara mus TF lentivirus Set kan programmera om musen embryonala fibroblaster (MEFs) till inducerad (IPS) pluripotenta stamceller. Här kan vi visa protokollet för DOX-inducerbara uttryck för musen omprogrammering transkriptionsfaktorer Oct4 till Sox2, Klf4 och c-Myc skapa iPS kolonier som uttrycker gemensamma MES pluripotens markörer.
Tags
Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set
