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Generering av inducerade pluripotenta stamceller genom att programmera om musen Embryonala fibroblasts med en fyra transkriptionsfaktor, Doxycyklin inducerbara Lentiviral Transduction System
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
DOI:

11:48 min

November 13, 2009

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Chapters

  • 00:00Title
  • 00:09Introduction
  • 00:30Viral Transduction of Mouse Embryonic Fibroblasts
  • 01:25Doxycycline Induced Reprogramming
  • 03:18Immunocytochemical Analysis of Transduction Efficiency
  • 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
  • 07:39Immunocytochemical Analysis for Pluripotency
  • 09:14Pluripotency Marker Analysis
  • 10:31Conclusion

Summary

Automatic Translation

Den Stemgent Dox inducerbara mus TF lentivirus Set kan programmera om musen embryonala fibroblaster (MEFs) till inducerad (IPS) pluripotenta stamceller. Här kan vi visa protokollet för DOX-inducerbara uttryck för musen omprogrammering transkriptionsfaktorer Oct4 till Sox2, Klf4 och c-Myc skapa iPS kolonier som uttrycker gemensamma MES pluripotens markörer.

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