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为核型分析人类胚胎干细胞的染色体蔓延制备
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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
Please note that all translations are automatically generated.
Click here for the English version.
为核型分析人类胚胎干细胞的染色体蔓延制备
DOI:
10.3791/1512-v
•
10:42 min
•
September 04, 2009
•
Priscila B. Campos
,
Rafaela C. Sartore
,
Stacie N. Abdalla
,
Stevens K. Rehen
1
Institute of Biomedical Sciences
,
Federal University of Rio De Janeiro-UFRJ
Chapters
00:00
Title
00:03
Introduction
00:57
Treating the Cells with Colecemid
01:46
Fixing the Cells
07:00
Washing the Cells
08:11
Preparing the Slides
09:14
Analyzing the Slides
Summary
Automatic Translation
English (Original)
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Automatic Translation
核型分析是一种简单而有用的技术,广泛用于检测遗传变异。在这里,我们描述了一个一步一步监测文化在保持这些细胞的染色体状态的人类胚胎干细胞的染色体蔓延的准备协议。
Tags
Chromosomal Spread Preparation
Human Embryonic Stem Cells
Karyotyping
Stable Diploid Karyotype
Chromosomal Anomalies
Culture Conditions
Enzymatic Dissociation
Chemical Dissociation
Manual Dissection
Feeder Cells
Genetic Integrity
Cellular Physiology
Embryogenesis Studies
Drug Testing
Therapeutic Purposes
Chromosomal Changes
Carcinogenesis
Chromosome Spreads
G-banding
FISH
SKY Techniques
CGH Techniques
Translocations
Aneuploidies
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