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为核型分析人类胚胎干细胞的染色体蔓延制备
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
DOI:

10:42 min

September 04, 2009

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Chapters

  • 00:00Title
  • 00:03Introduction
  • 00:57Treating the Cells with Colecemid
  • 01:46Fixing the Cells
  • 07:00Washing the Cells
  • 08:11Preparing the Slides
  • 09:14Analyzing the Slides

Summary

Automatic Translation

核型分析是一种简单而有用的技术,广泛用于检测遗传变异。在这里,我们描述了一个一步一步监测文化在保持这些细胞的染色体状态的人类胚胎干细胞的染色体蔓延的准备协议。

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