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使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
JoVE Journal
Biology
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JoVE Journal
Biology
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Please note that all translations are automatically generated.
Click here for the English version.
使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
DOI:
10.3791/1662-v
•
12:55 min
•
January 07, 2010
•
Adam M. McCoy
,
Michelle L. Collins
,
Luis A. Ugozzoli
1
Gene Expression Division
,
Bio-Rad Laboratories, Inc.
Chapters
00:00
Title
00:44
Introduction
02:07
Preparing the Mouse Embryonic Fibroblasts
04:00
The 96-well Electroporation Plates
05:20
Electroporating the MEFs in 96-well Plates
07:49
Electroporating the MEFs in Cuvettes
10:11
Epifluorescence Microscopy Images of Transfected Cells
12:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此过程说明如何使用基因脉冲MXcell电系统,以迅速和容易地识别小鼠胚胎成纤维细胞(MEFs)或其他主要细胞的最佳电条件。故障排除注意事项还讨论了相关的视频。
Tags
Gene Pulser MXcell Electroporation System
Transfect
Primary Cells
High Efficiency
Plasmid DNA
SiRNA
Electroporation Buffer
Bio-Rad
Nucleic Acids
Mammalian Cells
Difficult-to-transfect Cells
Stem Cells
Electroporation Conditions
Optimization
96-well Electroporation Plates
Electroporation Cuvettes
Electroporation Efficiency
Success Or Failure Of Electroporation Experiments
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