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使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
JoVE Journal
Biology
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JoVE Journal Biology
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
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使用基因脉冲MXcell电穿孔系统主细胞的转染效率高

DOI:
10.3791/1662-v

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12:55 min

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January 07, 2010

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, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc.

Chapters

  • 00:00Title
  • 00:44Introduction
  • 02:07Preparing the Mouse Embryonic Fibroblasts
  • 04:00The 96-well Electroporation Plates
  • 05:20Electroporating the MEFs in 96-well Plates
  • 07:49Electroporating the MEFs in Cuvettes
  • 10:11Epifluorescence Microscopy Images of Transfected Cells
  • 12:26Conclusion

Summary

Automatic Translation

Automatic Translation

此过程说明如何使用基因脉冲MXcell电系统，以迅速和容易地识别小鼠胚胎成纤维细胞（MEFs）或其他主要细胞的最佳电条件。故障排除注意事项还讨论了相关的视频。

Tags

Gene Pulser MXcell Electroporation SystemTransfectPrimary CellsHigh EfficiencyPlasmid DNASiRNAElectroporation BufferBio-RadNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsElectroporation ConditionsOptimization96-well Electroporation PlatesElectroporation CuvettesElectroporation EfficiencySuccess Or Failure Of Electroporation Experiments

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