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获得血细胞从夏威夷短尾鱿鱼
Euprymna scolopes
和观察他们坚持共生和非共生细菌
JoVE Journal
Biology
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JoVE Journal
Biology
Obtaining Hemocytes from the Hawaiian Bobtail Squid
Euprymna scolopes
and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
获得血细胞从夏威夷短尾鱿鱼
Euprymna scolopes
和观察他们坚持共生和非共生细菌
DOI:
10.3791/1714-v
•
05:29 min
•
February 11, 2010
•
Andrew J. Collins
,
Spencer V. Nyholm
1
Department of Molecular and Cell Biology
,
University of Connecticut
Chapters
00:00
Title
00:37
Introduction
01:27
Hemolymph Extraction from Euprymna scolopes
02:43
Hemocyte Preparation and Staining
04:23
Visualization of Hemocytes and Associated Bacteria
04:56
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
该视频将演示如何从夏威夷短尾乌贼的血细胞(血细胞),<em> Euprymna scolopes</em>细胞生物和细菌粘附实验使用。血细胞将用荧光染料染色和GFP标记的细菌接触到。
Tags
Hemocytes
Hawaiian Bobtail Squid
Euprymna Scolopes
Adherence
Symbiotic Bacteria
Non-symbiotic Bacteria
Immune System
Molecular Signaling
Beneficial Bacteria
Co-evolution
Eukaryotes
Microbiota
Model System
Animal Development
Macrophage-like Hemocytes
Innate Immune System
Establishment
Maintenance
Protocol
Anesthetized
Hemolymph Collection
Cephalic Blood Vessel
Chambered Glass Coverslips
Green Fluorescent Protein-labeled Symbiotic Vibrio Fischeri
Non-symbiotic Vibrio Harveyi
Fluorescent Dye
Cell Tracker Orange
Visualized
Fluorescent Microscopy
Article
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