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收获的小鼠精子和人工授精
JoVE Journal
Biology
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JoVE Journal
Biology
Harvesting Sperm and Artificial Insemination of Mice
Please note that all translations are automatically generated.
Click here for the English version.
收获的小鼠精子和人工授精
DOI:
10.3791/184-v
•
10:00 min
•
April 28, 2007
•
Amanda R. Duselis
,
Paul B. Vrana
1
Dept. of Biological Chemistry
,
University of California, Irvine (UCI)
Chapters
00:06
Introduction
01:09
Tools and Reagents
02:17
Dissection procedure
04:43
Removal of epididymis
07:58
Visualization of sperm
08:20
Insemination of females
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此协议表明,从男性的睾丸中提取精子,然后inseminating雌性小鼠的方法。此过程需要精确的时间是在发育的研究,以及转基因工作时非常有用。
Tags
Sperm Harvesting
Artificial Insemination
Mice
Peromyscus Species
Research
Model
Protocol
Testes Extraction
Sperm Isolation
Milk Mixture
Female Reproductive Tract
Fertilization
Embryo Development
Embryo Timing
Rodent Species
Mating Assessment
Transgenic Work
Reduced Animal Usage
Peromyscus Sy
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