Journal
/
/
嗨 - C:一个方法研究基因组的三维架构。
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
DOI:

22:27 min

May 06, 2010

, , , , , , ,

Chapters

  • 00:00Title
  • 01:03Introduction
  • 02:07Crosslinking, Digestion, Marking of DNA Ends, and Blunt-End Ligation
  • 09:46Shearing and Size Selection
  • 12:03Biotin Pull-Down and Paired End Sequencing
  • 16:38Representative Hi-C Results
  • 21:32Conclusion

Summary

Automatic Translation

的HI - C的方法,允许不带偏见,全基因组鉴定(1)染色质相互作用。您好- C的夫妇接近结扎和大规模并行测序。由此产生的数据可以用来研究基因组架构在多尺度的初步结果确定,如染色体领土,染色质开放式和封闭式的隔离,在megabase规模和染色质结构的特点。

Related Videos

Read Article