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Biology
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でアニマルキャップの移植(ACT)アッセイを用いて組織の決定
アフリカツメガエル</em
JoVE Journal
Biology
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JoVE Journal
Biology
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in
Xenopus laevis
Please note that all translations are automatically generated.
Click here for the English version.
でアニマルキャップの移植(ACT)アッセイを用いて組織の決定
アフリカツメガエル</em
DOI:
10.3791/1932-v
•
11:00 min
•
May 16, 2010
•
Andrea S. Viczian
,
Michael E. Zuber
1
Department of Ophthalmology
,
SUNY Upstate Medical University
Chapters
00:00
Title
00:59
Introduction
01:24
Setting Up for the ACT Assay
03:07
Preparing for Microinjection
05:18
Microinjection
06:30
Isolating Animal Caps from Injected Embryos
07:49
Transplantation of Caps onto Flank
09:53
Animal Cap Transplant Assay Representative Results
10:34
Conclusion
Summary
Automatic Translation
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アニマルキャップを過剰発現する遺伝子産物(s)を開発の側面に移植されています<em>アフリカツメガエル</em>胚組織が決定されているかどうかを確立するため。
Tags
Animal Cap Transplant Assay
Xenopus Laevis
Proteins
Embryonic Development
Cell Fate Determination
Noggin Overexpression
Eye Formation
Complex Phenotype
Eye-specific Role
Cell Fate Determination Assay
Gene Products
Eye Field Transcription Factors (EFTFs)
Retinal Cell Fate
Gene Product Identification
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