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Biomolecolare fluorescenza complementazione (BiFC) Assay per interazione proteina-proteina nelle cellule Cipolla Utilizzando la Helios Gun Gene
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Biomolecolare fluorescenza complementazione (BiFC) Assay per interazione proteina-proteina nelle cellule Cipolla Utilizzando la Helios Gun Gene

Article DOI: 10.3791/1963-v • 10:39 min • June 12th, 2010
June 12th, 2010 •
1, 1
1Dept. Of Cell Biology and Molecular Genetics, University of Maryland

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In questo articolo viene illustrato come utilizzare correttamente il BioRad Helios Gene Gun per introdurre DNA plasmidico in cellule epidermiche di cipolla e come test per interazioni proteina-proteina in cellule di cipolla in base al principio della complementazione bimolecolare fluorescenza (BiFC)

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Biologia Vegetale Numero 40 biomolecolare Complementazione fluorescenza (BiFC) bombardamento di particelle interazione proteina-proteina le cellule cipolla Helios Gun Gene
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