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双分子荧光互补(附设)检测蛋白质相互作用的洋葱细胞使用的Helios基因枪
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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
Please note that all translations are automatically generated.
Click here for the English version.
双分子荧光互补(附设)检测蛋白质相互作用的洋葱细胞使用的Helios基因枪
DOI:
10.3791/1963-v
•
10:39 min
•
June 12, 2010
•
Courtney A. Hollender
,
Zhongchi Liu
1
Dept. Of Cell Biology and Molecular Genetics
,
University of Maryland
Chapters
00:00
Title
00:57
Introduction
01:24
Preparing for Bombarding the Cells
03:32
Bombardment
07:16
Observation
08:50
Representative Images of the BiFC Assay in Onion Cells Transfected with the Helios Gene Gun
10:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了如何正确使用BioRad公司赫利奥斯基因枪引入洋葱表皮细胞的质粒DNA,以及如何测试基于双分子荧光互补的原则(附设)洋葱细胞中的蛋白质 - 蛋白质相互作用
Tags
Bimolecular Fluorescence Complementation
BiFC Assay
Protein-protein Interaction
Onion Cells
Helios Gene Gun
Gene Function
Fluorescent Protein
GFP
Plant Researchers
Onion Epidermal Cells
Plasmid DNA
Arabidopsis Thaliana
SEUSS
LEUNIG HOMOLOG
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