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Neuroscience
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在斑马鱼后脑细胞标记和成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Labeling and Imaging Cells in the Zebrafish Hindbrain
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼后脑细胞标记和成像
DOI:
10.3791/1976-v
•
09:17 min
•
July 25, 2010
•
Pradeepa Jayachandran
,
Elim Hong
,
Rachel Brewster
1
Department of Biological Sciences
,
University of Maryland, Baltimore County
,
2
Center for Neuroscience
,
Children’s National Medical Center
Chapters
00:05
Title
00:53
Introduction
01:27
Microinjection in the Zebrafish Hindbrain
03:56
Imaging mGFP-labeled Vibratome Sections
06:26
Live Imaging
07:49
Imaging Cells in the Hindbrain
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
理解,塑造了早期胚胎的形态发生过程的关键是对高分辨率图像细胞的能力。我们在这里描述膜有针对性的绿色荧光蛋白标签在整个斑马鱼的胚胎细胞的单细胞或小群的技术。
Tags
Labeling
Imaging Cells
Zebrafish Hindbrain
High Resolution
Plasmid DNA
Mosaic Expression
Visualization
Green Fluorescent Protein (mGFP)
Neurulation
Confocal Microscope
Vibratome Sectioning
Antibody Labeling
Time-lapse Confocal Microscopy
Neural Tube Formation
Cellular Behaviors
Hindbrain Region
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