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Immunology and Infection
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迁移人T淋巴细胞分离,培养和分析<em在体外</em
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JoVE Journal
Immunology and Infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
迁移人T淋巴细胞分离,培养和分析<em在体外</em
DOI:
10.3791/2017-v
•
08:39 min
•
June 01, 2010
•
Craig T. Lefort
,
Minsoo Kim
1
Center for Vaccine Biology and Immunology
,
University of Rochester
Chapters
00:00
Title
00:37
Introduction
01:31
Isolation of Human T Lymphocytes
02:57
Culture of Human T Lymphocytes
05:04
In Vitro Lymphocyte Migration Assay
06:15
Capturing an Image Sequence Using NIS Elements Software
07:20
Representative Results
08:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
T淋巴细胞的迁移过程中出现归巢淋巴器官,从血管中退出,进入周围组织。在这里,我们描述了一个可以用来分析T淋巴细胞迁移的协议<em>在体外</em>。
Tags
T Lymphocyte Isolation
Culture
Migration Analysis
Integrins
Ligands
Extracellular Matrix Proteins
Cell Leading Edge
Uropod
Leukocyte Recruitment
Autoimmune Diseases
Inflammatory Diseases
In Vitro System
Two-dimensional Substrate
Intercellular Adhesion Molecule-1 (ICAM-1)
Integrin LFA-1
Stromal Cell-derived Factor-1 (SDF-1)
Migratory Velocity
Integrin Blockade
Actomyosin Machinery
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