Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Please note that all translations are automatically generated.
DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.