JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在激活人类细胞中的基因表达的动态检测组蛋白修饰染色质免疫沉淀(ChIP)
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
在激活人类细胞中的基因表达的动态检测组蛋白修饰染色质免疫沉淀(ChIP)
DOI:
10.3791/2053-v
•
13:20 min
•
July 29, 2010
•
Lauren J. Buro
,
Shaili Shah
,
Melissa A. Henriksen
1
Department of Biology
,
University of Virginia
Chapters
00:05
Title
01:11
Introduction
01:33
Crosslinking
03:32
Preparing Chromatin
05:37
Chromatin IP (ChIP)
07:50
Collecting Immunocomplexes
10:01
Recovering the DNA
11:45
Representative Results
12:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本协议描述了如何染色质免疫沉淀(ChIP)是用来研究的动态改变,调节信号转导通路诱导转录的染色质模板。
Tags
Chromatin Immunoprecipitation
ChIP
Histone Modification
Activated Gene Expression
Human Cells
STAT Transcription Factors
Extracellular Ligands
Phosphorylation
Tyrosine Residue
Nucleus
Target Genes
Growth
Differentiation
Homeostasis
Immune Response
Dysregulation
Human Disease
Cancers
Autoimmune Diseases
Chromatin Template
ATP-dependent Complexes
Covalent Histone Modifications
DNA Methylation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
从人类胚胎干细胞的染色质免疫沉淀
使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
机器人技术和植物组织的基因表达研究的动态图像分析
使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
等位基因特异性基因表达检测,测试的遗传协会的功能基础
克隆形成含量:贴壁细胞
使用早期的小鼠胚胎组织特异性基因的染色质免疫沉淀检测
在体外
重组活跃
T。谷盗
端粒酶
染色质免疫沉淀(ChIP)使用
果蝇
组织
作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列
Read Article