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Kromatin Immunoprecipitation (chip) för analys Dynamisk Histon Ändring i aktiverade genuttryck i humana celler
JoVE Journal
Biology
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JoVE Journal Biology
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
DOI:

13:20 min

July 29, 2010

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Chapters

  • 00:05Title
  • 01:11Introduction
  • 01:33Crosslinking
  • 03:32Preparing Chromatin
  • 05:37Chromatin IP (ChIP)
  • 07:50Collecting Immunocomplexes
  • 10:01Recovering the DNA
  • 11:45Representative Results
  • 12:44Conclusion

Summary

Automatic Translation

Detta protokoll beskriver hur kromatin immunoprecipitation (chip) används för att studera dynamiska förändringar i kromatin mall som reglerar transkription inducerad av en signaltransduktion väg.

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