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1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 3Department of Pathology,The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children’s Research Institute,Royal Children’s Hospital, 5Department of Pediatrics,The University of Melbourne
Chapters
- 00:00Title
- 00:05Introduction
- 01:42Tissue Preparation
- 02:29Immunofluorescence Staining
- 05:19Microscopy
- 07:02Analysis
- 08:24Conclusion
डीएनए γH2AX foci के आधार पर डबल भूग्रस्त टूटता quantitation एक अमूल्य उपकरण बन गया है, ऊतक radiosensitivity और विकिरण संशोधित यौगिकों के प्रभाव के मूल्यांकन के लिए विकिरण जीव विज्ञान में, विशेष रूप से,. यहाँ हम γH2AX foci के ऊतकों के नमूनों में quantitation के लिए एक immunofluorescence परख का उपयोग प्रदर्शित करता है.
Tags
QuantitationγH2AX FociDNA Double-strand BreaksEndogenous ProcessesExogenous FactorsIonizing RadiationRadiomimetic CompoundsH2A Histone VariantSerine-139 ResiduePhosphorylationPI3K Family Of ProteinsATM KinaseATR KinaseNuclear γH2AX FociImmunofluorescence MicroscopyMarker Specificity And SensitivityBiomedical ResearchRadiation BiologyNuclear MedicineCell Culture SystemsIonizing Radiation-induced DSBsCellular RadiosensitivityRadiation-modifying CompoundsDSB-inducing Compounds
