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Journal
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Biology
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RhoC GTPase Assay Activation
JoVE Journal
Biology
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JoVE Journal
Biology
RhoC GTPase Activation Assay
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RhoC GTPase Assay Activation
DOI:
10.3791/2083-v
•
09:58 min
•
August 22, 2010
•
Michelle Lucey
,
Heather Unger
,
Kenneth L. van Golen
1
Department of Biological Sciences, The Center for Translational Cancer Research
,
University of Delaware
Chapters
00:00
Title
00:48
Introduction
01:25
Preparation of GST-fusion Protein
04:41
GST-RBD Pull Down Assay
09:01
Representative Results for a RhoC GTPase Activation Assay
09:34
Conclusion
Summary
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Automatic Translation
פרוטוקול זה מנצל לנתץ assay כדי לקבוע את רמות של GTPase RhoC פעיל בתוך התאים.
Tags
RhoC GTPase
RhoA GTPase
Activation Assay
Homology
Reagents
Techniques
Metastatic Cancer Cells
Rho Activation Assay
GST-Rho Binding Domain Fusion Protein
Active RhoC GTPase
Total Protein
GTP Bound
GDP Bound
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RhoC-specific Antibody
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