Journal
/
Biology
/
RhoC的GTP酶激活法
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
RhoC GTPase Activation Assay
Please note that all translations are automatically generated. Click here for the English version.

RhoC的GTP酶激活法

DOI:
10.3791/2083-v

•

09:58 min

•

August 22, 2010

•

, ,
1Department of Biological Sciences, The Center for Translational Cancer Research,University of Delaware

Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:25Preparation of GST-fusion Protein
  • 04:41GST-RBD Pull Down Assay
  • 09:01Representative Results for a RhoC GTPase Activation Assay
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

该协议利用上拉下来检测，以确定细胞内的活动中RhoC GTP酶的水平。

Tags

RhoC GTPaseRhoA GTPaseActivation AssayHomologyReagentsTechniquesMetastatic Cancer CellsRho Activation AssayGST-Rho Binding Domain Fusion ProteinActive RhoC GTPaseTotal ProteinGTP BoundGDP BoundCommercial VersionsCommercial KitsRhoC-specific Antibody

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

专访：糖脂抗原提呈CD1d及NKT细胞活化治疗潜力

自由表现动物的神经回路活化的录音

主动脉环含量

分离和<em在体外激活线虫精子

激活细胞凋亡的细胞色素的细胞质显微注射 C</em

细胞内复性检测

酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析

单分子成像的基因调控<em在体内</em使用转位激活裂解（CoTrAC）

线虫趋含量的

比较GTP酶结合蛋白的使用竞争性试验的亲和

Read Article