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Chapters
- 00:05Title
- 00:51Introduction
- 01:08Protein Overexpression
- 02:48Harvesting and Extracting the Cells
- 04:58Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
- 06:46Hexahistidine Tag Affinity Purification by IMAC
- 08:23Gel Filtration
- 09:44Representative Results from MexB Purification
- 10:23Conclusion
I detta protokoll visar vi uttrycket lösningsgörande, och rening av recombinantly uttryckt membranprotein, MexB som ett lösligt protein rengöringsmedel komplex. MexB är en multidrogresistensgenen membran transportör från de opportunistiska bakteriell patogen Pseudomonas aeruginosa.
Tags
Membrane TransporterMexB Multidrug Resistance ProteinMultidrug ResistanceDrug Efflux PumpsPseudomonas AeruginosaTripartite ComplexesInner Membrane Proton-drug AntiporterOuter Membrane ChannelPeriplasmic Linker ProteinTransmembrane ProteinsProtein Detergent Complex (PDC)Detergent SolubilizationPurification
