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İfade, Deterjan çözünürleştirme ve Membran Transporter, MexB ilaca Direnç Protein Saflaştırılması
JoVE Journal
Biology
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JoVE Journal Biology
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
DOI:

10:43 min

December 03, 2010

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Chapters

  • 00:05Title
  • 00:51Introduction
  • 01:08Protein Overexpression
  • 02:48Harvesting and Extracting the Cells
  • 04:58Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
  • 06:46Hexahistidine Tag Affinity Purification by IMAC
  • 08:23Gel Filtration
  • 09:44Representative Results from MexB Purification
  • 10:23Conclusion

Summary

Automatic Translation

Bu protokolde, çözünebilir bir protein deterjan kompleksi olarak, ifade, çözünmüş ve recombinantly ifade membran proteini, MexB arıtma göstermektedir. MexB fırsatçı bakteriyel patojen Pseudomonas aeruginosa bir çok ilaca direnç membran taşıyıcı.

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