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Chapters
- 00:05Title
- 00:51Introduction
- 01:08Protein Overexpression
- 02:48Harvesting and Extracting the Cells
- 04:58Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
- 06:46Hexahistidine Tag Affinity Purification by IMAC
- 08:23Gel Filtration
- 09:44Representative Results from MexB Purification
- 10:23Conclusion
In diesem Protokoll zeigen wir den Ausdruck, Solubilisierung und Reinigung von rekombinant exprimierten Membranprotein, MexB, wie ein lösliches Protein Waschmittel komplex. MexB ist ein Multidrug-Resistenz-Membran-Transporter aus der opportunistische bakterielle Erreger Pseudomonas aeruginosa.
Tags
Membrane TransporterMexB Multidrug Resistance ProteinMultidrug ResistanceDrug Efflux PumpsPseudomonas AeruginosaTripartite ComplexesInner Membrane Proton-drug AntiporterOuter Membrane ChannelPeriplasmic Linker ProteinTransmembrane ProteinsProtein Detergent Complex (PDC)Detergent SolubilizationPurification
