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投诉矩阵量化细胞收缩的准备工作

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Preparation of Complaint Matrices for Quantifying Cellular Contraction

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投诉矩阵量化细胞收缩的准备工作

DOI:

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11:38 min

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December 14, 2010

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Yvonne Aratyn-Schaus, Patrick W. Oakes, Jonathan Stricker, Stephen P. Winter, Margaret L. Gardel²

¹Institute for Biophysical Dynamics,University of Chicago, ²Physics Department – James Franck Institute,University of Chicago, ³Interdisciplinary Scientist Training Program,University of Chicago

Chapters

00:05Title
01:15Coverslip Surface Activation
03:00Polyacrylamide (PAA) Gel Preparation
05:07Extracellular Matrix (ECM) Protein Crosslinking to the PAA Gel
07:30Confocal Imaging
09:47Representative Results for Quantifying Cellular Contraction
11:15Conclusion

Summary

Automatic Translation

在这段视频中，我们展示了用于制造兼容，细胞外基质（ECM）适用于细胞培养镀膜基片的实验技术，适合牵引力显微镜观察细胞的行为流脑刚度的影响。

Tags

Complaint Matrices Quantifying Cellular Contraction Cellular Adhesion Extracellular Matrix (ECM)Focal Adhesions F-actin Cytoskeleton Mechanical Forces Cell Migration ECM Remodeling Cell Culture Substrates Traction Force Microscopy (TFM)Substrate Deformations Cytoskeletal Organization Fluorescence Microscopy

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