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Bioengineering
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投诉矩阵量化细胞收缩的准备工作
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Preparation of Complaint Matrices for Quantifying Cellular Contraction
Please note that all translations are automatically generated.
Click here for the English version.
投诉矩阵量化细胞收缩的准备工作
DOI:
10.3791/2173-v
•
11:38 min
•
December 14, 2010
•
Yvonne Aratyn-Schaus
,
Patrick W. Oakes
,
Jonathan Stricker
,
Stephen P. Winter
,
Margaret L. Gardel
2
1
Institute for Biophysical Dynamics
,
University of Chicago
,
2
Physics Department – James Franck Institute
,
University of Chicago
,
3
Interdisciplinary Scientist Training Program
,
University of Chicago
Chapters
00:05
Title
01:15
Coverslip Surface Activation
03:00
Polyacrylamide (PAA) Gel Preparation
05:07
Extracellular Matrix (ECM) Protein Crosslinking to the PAA Gel
07:30
Confocal Imaging
09:47
Representative Results for Quantifying Cellular Contraction
11:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这段视频中,我们展示了用于制造兼容,细胞外基质(ECM)适用于细胞培养镀膜基片的实验技术,适合牵引力显微镜观察细胞的行为流脑刚度的影响。
Tags
Complaint Matrices
Quantifying Cellular Contraction
Cellular Adhesion
Extracellular Matrix (ECM)
Focal Adhesions
F-actin Cytoskeleton
Mechanical Forces
Cell Migration
ECM Remodeling
Cell Culture Substrates
Traction Force Microscopy (TFM)
Substrate Deformations
Cytoskeletal Organization
Fluorescence Microscopy
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