JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
異種哺乳動物細胞で発現したイオンチャネルの変異導入と機能解析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
異種哺乳動物細胞で発現したイオンチャネルの変異導入と機能解析
DOI:
10.3791/2189-v
•
15:28 min
•
October 01, 2010
•
Bartosz Balana
,
Natalie Taylor
,
Paul A. Slesinger
1
Clayton Foundation Laboratories for Peptide Biology
,
Salk Institute for Biological Studies
Chapters
00:00
Title
01:06
Introduction
01:44
Site Directed Mutagenesis and Preparation of Plasmid DNA for Transfection
03:09
Plating of Cells, Transient Transfection, and Imaging of Transfected Cells
05:00
Whole-cell Patch Clamping
12:57
Representative Analysis
14:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我々は、イオンチャンネルの機能上の単一の点変異の効果を研究する方法を説明します。
Tags
Mutagenesis
Functional Analysis
Ion Channels
Heterologously Expressed
Mammalian Cells
Point Mutation
GIRK Channels
G Protein-gated Inwardly Rectifying Potassium Channels
Excitability Of Neurons
G Protein-coupled Receptors
Muscarinic Receptor
Alcohol Activation
Amino Acid Mutation
CDNA Sequencing
Transfection
HEK293T Cells
Patch-clamp Electrophysiology
Potassium Currents
L257W Mutation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
アフリカツメガエルの卵母細胞で発現イオンチャネルのパッチクランプ記録
哺乳動物細胞での糖タンパク質からN -結合型糖鎖のパルス - チェイス分析
哺乳動物細胞におけるDNA二重鎖切断(DSB)修復の解析
イオンチャネル研究のためのパッチクランプおよび灌流のテクニックで表さ
アフリカツメガエル
卵母細胞
発現とHEK - 293T細胞における組換え電位依存性イオンチャネルの電気生理学的記録用に最適化されたトランスフェクション戦略
マイクロインジェクションによる哺乳動物細胞におけるmRNA核外輸送の速度論の解析
内タンパク質の定方向進化のための変異導入と機能選択プロトコル
E.大腸菌</em
哺乳動物細胞における細胞周期の位置の解析
培養細胞およびマウス組織から単離とミトコンドリアの機能解析
ナの機能的特徴<sup> +</sup> / H<sup> +</sup細胞膜でそれらを表現するためにプロトン殺す選択を使用した細胞内区画の>交換器
Read Article