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Journal
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Biology
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Mutagenese en Functionele analyse van ionenkanalen heteroloog Uitgedrukt in zoogdiercellen
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
Mutagenese en Functionele analyse van ionenkanalen heteroloog Uitgedrukt in zoogdiercellen
DOI:
10.3791/2189-v
•
15:28 min
•
October 01, 2010
•
Bartosz Balana
,
Natalie Taylor
,
Paul A. Slesinger
1
Clayton Foundation Laboratories for Peptide Biology
,
Salk Institute for Biological Studies
Chapters
00:00
Title
01:06
Introduction
01:44
Site Directed Mutagenesis and Preparation of Plasmid DNA for Transfection
03:09
Plating of Cells, Transient Transfection, and Imaging of Transfected Cells
05:00
Whole-cell Patch Clamping
12:57
Representative Analysis
14:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
We zullen zien hoe het effect van een enkele puntmutatie op de functie van een ionkanaal studie.
Tags
Mutagenesis
Functional Analysis
Ion Channels
Heterologously Expressed
Mammalian Cells
Point Mutation
GIRK Channels
G Protein-gated Inwardly Rectifying Potassium Channels
Excitability Of Neurons
G Protein-coupled Receptors
Muscarinic Receptor
Alcohol Activation
Amino Acid Mutation
CDNA Sequencing
Transfection
HEK293T Cells
Patch-clamp Electrophysiology
Potassium Currents
L257W Mutation
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