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原代细胞培养从果蝇原肠胚
JoVE Journal
Biology
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JoVE Journal Biology
Primary Cell Cultures from Drosophila Gastrula Embryos
DOI:

10:55 min

February 28, 2011

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Chapters

  • 00:05Title
  • 01:46Reagent Set-up and Preparation of Fly Cages
  • 02:52Collections of Synchronous Embryos
  • 03:36Collecting, Washing, Homogenizing Embryos, and Plating of Cells
  • 06:35Primary-Cell RNAi for Cells Grown in a 384-well Plate
  • 07:48Representative Images of Cultured Cells
  • 10:30Conclusion

Summary

Automatic Translation

我们提供一份详细的协议,准备原代细胞脱离<em> Drosophil</em>一个胚胎。能够进行有效的RNAi扰动,与其他分子生物学,生物化学和细胞成像方法,将允许各种问题加以解决,<em>果蝇</em>原代细胞。

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