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原代细胞培养从果蝇原肠胚
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JoVE Journal Biology
Primary Cell Cultures from Drosophila Gastrula Embryos
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原代细胞培养从果蝇原肠胚

DOI:
10.3791/2215-v

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10:55 min

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February 28, 2011

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, ,
1Department of Genetics,Harvard Medical School, 2Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 01:46Reagent Set-up and Preparation of Fly Cages
  • 02:52Collections of Synchronous Embryos
  • 03:36Collecting, Washing, Homogenizing Embryos, and Plating of Cells
  • 06:35Primary-Cell RNAi for Cells Grown in a 384-well Plate
  • 07:48Representative Images of Cultured Cells
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

我们提供一份详细的协议，准备原代细胞脱离<em> Drosophil</em>一个胚胎。能够进行有效的RNAi扰动，与其他分子生物学，生物化学和细胞成像方法，将允许各种问题加以解决，<em>果蝇</em>原代细胞。

Tags

Primary Cell CulturesDrosophila Gastrula EmbryosCell DissociationSingle Cell SuspensionCulture PlatesChamber SlidesCell DifferentiationCell MarkersGene KnockdownRNAiDsRNACulture MediumMolecular AnalysisBiochemical AnalysisCell Imaging AnalysisDifferentiated Muscle CellsNeuronal Cells

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