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酶联免疫斑点法（ELISPOT）：TH - 1细胞对微生物抗原的免疫应答的定量
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Immunology and Infection
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JoVE Journal Immunology and Infection
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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酶联免疫斑点法（ELISPOT）：TH - 1细胞对微生物抗原的免疫应答的定量

DOI:
10.3791/2221-v

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06:13 min

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November 23, 2010

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, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 00:58Coating the ELISPOT Plate
  • 01:45Cell Preparation
  • 02:08Setting up the Plate
  • 03:25Developing the ELISPOT Plate
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

可以通过使用的酶联免疫检测鉴定的微生物指标在特发性疾病的适应性免疫。

Tags

Enzyme-linked Immunospot AssayELISPOTCellular Immune ResponsesMicrobial AntigensTh-1 Immune ResponseDiagnostic ToolImmunosuppressed PopulationsHIV InfectionTransplantationSteroid UseTh1 Or Th2 Immune ResponsePeripheral BloodBronchoalveolar LavageCerebral Spinal FluidAscitesEpitopes Within Microbial ProteinsImmune Responses Over TimeDual Color ELISPOT AssaysCytokinesExtrapulmonary TuberculosisInfectious AntigensAutoimmunity

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