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Cryopreserving和恢复人类iPS细胞血清替代馈线中使用完整的基因敲除
JoVE Journal
Biology
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JoVE Journal
Biology
Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
Please note that all translations are automatically generated.
Click here for the English version.
Cryopreserving和恢复人类iPS细胞血清替代馈线中使用完整的基因敲除
DOI:
10.3791/2237-v
•
06:47 min
•
July 15, 2010
•
Kate Wagner
,
David Welch
1
GIBCO
,
Life Technologies
Chapters
00:04
Title
00:44
Introduction
01:31
Cryopreserving iPSCs
03:48
Thawing iPSCs
05:54
Representative Results
06:24
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
这一协议描述为cryopreserving淘汰赛SR冻存介质的人类iPS细胞,这些细胞恢复完整的基因敲除SR直属免费(KSR - FF)介质或进纸器为基础的基因敲除的SR介质的详细过程。
Tags
Cryopreserving
Recovering
Human IPS Cells
Complete KnockOut Serum Replacement
Feeder-free Medium
Reprogramming
Fibroblasts
Pluripotent Cells
Drug Discovery
Cell Therapy
Basic Research
GIBCO Media And Reagents
Knockout DMEM
Knockout Serum Replacement
Embryonic Stem Cell Growth
IPS Cell Culture
Feeder-free Culture
Knockout SR Growth Factor Cocktail
Traditional Culture Methods
Mouse Or Human Fibroblast Feeder Layers
Feeder-conditioned Medium
Labor-intensive
Hard To Scale
Undifferentiated Cells
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