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Chapters
- 00:02Introduction
- 01:24Preparing Spinal Cord Emulsion
- 08:43Loading Glass Syringes with Emulsion
- 12:37Injecting Rats with Spinal Cord Emulsion
- 15:51Monitoring Clinical Signs (CS)
- 17:31CS 0.0 – No Symptoms of EAE
- 18:50CS 0.5 – Distal Limp Tail
- 19:29CS 1.0 – Limp Tail
- 20:21CS 2.0 – Weakened Hindlimbs
- 21:59CS 3.0 – Moderate Hindlimb Paralysis
- 23:06CS 3.5 – Paralysis of Single Hindlimb
- 23:28CS 4.0 – Complete Hindlimb Paralysis
- 23:44CS 5.0 – Complete Hindlimb Paralysis and Incontinence
- 24:07Caring for Animals with EAE
- 24:53Conclusion
- 26:39End Credits
Deze video toont de inductie en de klinische scoren van een diermodel van multiple sclerose: chronisch-recidiverende experimentele auto-immune encephalomyelitis in DA ratten. De ziekte, veroorzaakt door het immuniseren van ratten met een emulsie met hele rat het ruggenmerg en de volledige Freund's adjuvans, presenteert klinische symptomen lijken op de menselijke ziekte.
Tags
InductionClinical ScoringChronic-Relapsing Experimental Autoimmune EncephalomyelitisMultiple SclerosisChronic Inflammatory DiseaseCentral Nervous SystemDemyelinationGlial ScaringBrainSpinal CordDemyelinated PlaquesParaparesisParalysisBlindnessIncontinenceExperimental Autoimmune Encephalomyelitis (EAE)MS ModelVaccinationRabiesViruses Grown On Rabbit Spinal CordsCNS DiseaseNon-human Primates Immunized With A CNS HomogenateSpecies/strainImmunizing AntigenLewis RatsMyelin Basic Protein In Emulsion With AdjuvantGuinea PigsDA RatsDA Rat Spinal CordComplete Freund’s AdjuvantAscending Flaccid Paralysis
