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Journal
/
Biology
/
在
完整的
果蝇
幼虫
体内的
亚细胞分辨率成像
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
In vivo
Imaging of Intact
Drosophila
Larvae at Sub-cellular Resolution
Please note that all translations are automatically generated.
Click here for the English version.
在
完整的
果蝇
幼虫
体内的
亚细胞分辨率成像
DOI:
10.3791/2249-v
•
17:51 min
•
September 10, 2010
•
Yao Zhang*
1,2
,
Petra Füger*
1
,
Shabab B. Hannan
2
,
Jeannine V. Kern
,
Bronwen Lasky
,
Tobias M. Rasse
1
Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research
,
University of Tübingen
,
2
Graduate School of Cellular and Molecular Neuroscience
,
University of Tübingen
Chapters
00:00
Title
00:05
Introduction
02:01
Preparing Larva
03:00
Assembly of the Imaging Chamber
04:36
Anesthetization of the Larva
07:48
Imaging
14:39
Recovery from Anesthetization
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了一种可靠的方法,麻醉和完整的成像<em>果蝇</em>幼虫。我们利用挥发性麻醉药氟烷允许重复成像,在亚细胞的分辨率和结构的重新鉴定几天<sup> 1</sup>。
Tags
In Vivo Imaging
Drosophila Larvae
Sub-cellular Resolution
Optical Imaging
Genetically Encoded Fluorophores
Genetic Tools
Transgenic Animal Lines
Anesthetize Larvae
Desflurane
Synaptic Populations
High Degree Of Anesthetization
Survival Rate
Time-points
Proteins Dynamics
Glutamate Receptor Subunit GluR-IIA
Endogenous Promoter
Exon Trap Line FasII-GFP
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