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Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Chapters
- 00:00Title
- 01:13Habituating Animals
- 01:29Eliciting Target-Specific Effector CTLs by Immunization (Day 0)
- 03:03Program Operant Test Session
- 03:16Harvesting Target Cells (Day 8)
- 05:32Treatment and Labeling of Target Cells
- 06:33Results: Operant Sensation Seeking Behavior
- 07:39Adoptive Transfer and Subsequent Harvest of Labeled Cells
- 08:06Conclusion
- 08:20Flow Cytometry and Data Analysis
- 08:36CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice
- 09:34Conclusion
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
Tags
Antigen Specific In Vivo Killing AssayCFSE Labeled Target CellsCarboxyfluorescein Diacetate Succinimidyl EsterIn Vivo InvestigationCell LabelingAdoptive TransferSurvivalKillingEpitope SpecificCD8+ T Cell CloneResponse QualityCytokine ProductionFunctional ExhaustionTCR SpecificitiesCell Mediated ImmunityResponding T CellsPeptide Specific T Cell ClonesBALB/c Mice
