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항원 특정 VIVO에서 죽이는 분석
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
DOI:

09:49 min

November 09, 2010

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Chapters

  • 00:00Title
  • 01:13Habituating Animals
  • 01:29Eliciting Target-Specific Effector CTLs by Immunization (Day 0)
  • 03:03Program Operant Test Session
  • 03:16Harvesting Target Cells (Day 8)
  • 05:32Treatment and Labeling of Target Cells
  • 06:33Results: Operant Sensation Seeking Behavior
  • 07:39Adoptive Transfer and Subsequent Harvest of Labeled Cells
  • 08:06Conclusion
  • 08:20Flow Cytometry and Data Analysis
  • 08:36CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice
  • 09:34Conclusion

Summary

Automatic Translation

대부분의 감염은 강력한 CTL 반응을 이끌어내는,하지만 가끔 응답 세포의 수량은 병원균 제어 상관 관계를하지 않습니다<sup> 1</sup>. CTL 품질 측정 중 하나는 특별히 죽이는 능력이있다<sup> 2</sup>. 대상 세포의 CFSE 라벨이 CTL 응답 품질을 조사하는 데 사용할 수 있습니다<em> 생체내에</em<sup> 3,4</sup>.

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