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Biology
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膜片钳和灌注技术研究离子通道的表达
爪蟾
卵母细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in
Xenopus
oocytes
Please note that all translations are automatically generated.
Click here for the English version.
膜片钳和灌注技术研究离子通道的表达
爪蟾
卵母细胞
DOI:
10.3791/2269-v
•
10:19 min
•
January 10, 2011
•
Junqiu Yang
,
Kelli Delaloye
,
Urvi S. Lee
,
Jianmin Cui
1
Department of Energy, Environmental & Chemical Engineering
,
Washington University in St. Louis
,
2
Department of Biomedical Engineering
,
Washington University in St. Louis
,
3
Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center
,
Washington University in St. Louis
Chapters
00:05
Title
00:45
Introduction
01:10
Injecting mRNA into Oocytes
01:51
Preparing the Perfusion System
04:26
Preparing the Ag/AgCl Wire and the Data Acquisition Equipment
05:33
Stripping the Vitelline Membrane from the Oocyte
06:14
Preparing the Patch Pipettes
07:06
Obtaining an Inside-Out Patch
08:08
Patch Clamping
08:41
Recording of Wild-Type BK Channels on and Oocyte Membrane Patch
09:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
BK通道的离子电流是利用膜片钳技术记录。 BK通道均以<em>爪蟾卵母细胞</em注射信使RNA。在膜片钳记录细胞内的解决方案是由灌注系统控制。
Tags
Patch Clamp
Perfusion Techniques
Ion Channels
Xenopus Oocytes
BK Channels
Activation
Structure-function Relationship
Neurotransmitter Receptors
Smooth Muscle Contraction
Hair Cell Frequency Tuning
Neurotransmitter Release
Membrane Depolarization
Intracellular Ca2+ And Mg2+
Messenger RNA Injection
Incubation
Inside-out Configuration
Single Or Multiple Channels
Recording Currents
Controlled Voltage
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