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封闭的系统使用透氧材料科技HYPERStack船只的细胞培养协议
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
Please note that all translations are automatically generated.
Click here for the English version.
封闭的系统使用透氧材料科技HYPERStack船只的细胞培养协议
DOI:
10.3791/2499-v
•
11:48 min
•
November 29, 2010
•
Kim Titus*
1
,
Vitaly Klimovich*
2
,
Mark Rothenberg
,
Pilar Pardo*
2
,
Allison Tanner*
3
,
Greg Martin
1
Business Development
,
Corning Life Science
,
2
Applications
,
Corning Life Science
,
3
Product Development
,
Corning Life Science
Chapters
00:06
Title
01:06
HYPERStack Vessel Component Summary
02:44
Vessel Preparation
05:34
Isolation Procedure to Lock Liquid into the Layers
07:09
Harvest Protocol
10:37
Gas Diffusion Results through Gas Permeable Film used in the HYPERStack Vessels
11:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
介绍到高产贴壁细胞培养使用康宁HYPERStack船只及配件的技术,协议和处理。该协议将展示如何使用目前的叠板产品的封闭系统中增加细胞收集船只。
Tags
Closed System Cell Culture
HYPERStack Vessels
Gas Permeable Material Technology
Large Volume Adherent Cell Culture
Stacked Plate Cell Growth Products
Microcarrier Beads
Scale Up
Higher Cell Yield
Gas Exchange
Internal Headspace Elimination
Space Optimization
Cell Therapy
Vaccine Production
Closed System Protocol
Cell And Reagent Addition/removal
Tubing
Media Bags
Gas Diffusion
Metabolic Needs Of Cells
Harvesting Methods
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